Doubled Haploid Technology

• A doubled haploid (DH) is a genotype formed when haploid cells undergo chromosome doubling. Artificial production of doubled haploids is important in plant breeding.
• For doubled haploids to become a valuable breeding technique, it is necessary that success of doubled haploid production is not genotype dependent, the protocol used is efficient in time and cost.
• Benefits of this technology are ,Double Haploid technology creates true-breeding lines in a single step, Combined DH & molecular marker technologies  result in increased rates of genetic gain,  Facilitates multiple trait stacking/pyramiding, Increases efficiency & probability of successful product development ,Reduces time to market.
• Tobacco, rapeseed, and barley are the most responsive species for doubled haploid production. Doubled haploid methodologies have now been applied to over 250 species.