Tuesday, August 14, 2012

Doubled Haploid Technology

  • A doubled haploid (DH) is a genotype formed when haploid cells undergo chromosome doubling. Artificial production of doubled haploids is important in plant breeding.
  • For doubled haploids to become a valuable breeding technique, it is necessary that success of doubled haploid production is not genotype dependent, the protocol used is efficient in time and cost.
  • Benefits of this technology are ,Double Haploid technology creates true-breeding lines in a single step, Combined DH & molecular marker technologies  result in increased rates of genetic gain,  Facilitates multiple trait stacking/pyramiding, Increases efficiency & probability of successful product development ,Reduces time to market.
  • Tobacco, rapeseed, and barley are the most responsive species for doubled haploid production. Doubled haploid methodologies have now been applied to over 250 species.

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