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Wednesday, June 13, 2012


  • Elicitor induction of phenylpropanoid metabolism was investigated in suspension-cultured cells of the fast-growing poplar hybrid (Populus trichocarpa Torr. & Gray × Populism deltoides Marsh) H11-11. Treatment of cells with polygalacturonic acid lyase or two fungal elicitors resulted in rapid and transient increases in extractable l-phenylalanine ammonia lyase and coumarate:coenzyme  ligase enzyme activities.
  • The substrate specificity of the inducible coumarate:coenzyme A ligase enzyme activity appeared to differ from substrate specificity of coumarate:coenzyme a ligase enzyme activity in untreated control cells.
  • Large and transient increases in the accumulation of phenylalanine ammonia-lyasecoumarate: coenzyme A ligase mRNAs preceded the increases in enzyme activities and were detectable by 30 minutes after the start of elicitor treatment

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