- Elicitor induction of phenylpropanoid metabolism was investigated in suspension-cultured cells of the fast-growing poplar hybrid (Populus trichocarpa Torr. & Gray × Populism deltoides Marsh) H11-11. Treatment of cells with polygalacturonic acid lyase or two fungal elicitors resulted in rapid and transient increases in extractable l-phenylalanine ammonia lyase and coumarate:coenzyme ligase enzyme activities.
- The substrate specificity of the inducible coumarate:coenzyme A ligase enzyme activity appeared to differ from substrate specificity of coumarate:coenzyme a ligase enzyme activity in untreated control cells.
- Large and transient increases in the accumulation of phenylalanine ammonia-lyasecoumarate: coenzyme A ligase mRNAs preceded the increases in enzyme activities and were detectable by 30 minutes after the start of elicitor treatment
Elicitor
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